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Typically primers greater than 20 nucleotides in length anneal for 1030. Web The NEB Tm calculator should be used to determine the annealing temperature when using Phusion.


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Web Unlike other calculators the NEB Tm Calculator takes buffer components that affect melting temperatures into consideration when calculating the best annealing.

. Web The calculator calculates recommended T m melting temperature of primers and PCR annealing temperature based on the primer pair sequence primer concentration and. Use NEBioCalculator to convert DNA. Web Use Tm Calculator to calculate optimum annealing temperature for PCR primers when using NEB polymerases and buffers.

Web Choose a DNA RNA genome editing qPCR calculator from NEB a leader in production and supply of reagents for the life science industry.

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